酵素化學實驗 Index 索 引 S-Z | |||
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S | |||
Salting out 鹽析, 69, 114-117, 123, 138 Sandwich method, ELISA 三明治法, 95 Sandwich method (gel drying) 膠片乾燥三明治法, 155, 216 Sarin [商品名] 沙林毒氣, 82 Schiff's reagent, Schiff 氏試劑, 153-154, 214 SDS (sodium dodecyl sulfate) 硫酸十二酯鈉, 143, 146, 154, 207-208 SDS-PAGE, SDS 膠體電泳, 8-13, 138, 149-153, 166, 207-209 two-dimensional electrophoresis 二次元電泳, 12-13, 155, 166 microanalysis 微量分析技術, 161, 166-167 protein transfer 蛋白質轉印, 155, 161, 166, 207-209, 217-218 Second order reaction 二級反應, 80 Secondary antibody 二次抗體, 95, 218-220 Secondary bonds 二級鍵, 55, 68, 86 Secondary structure 二級構造, 64, 65-66, 159, 226 Sedimentation coefficient 沉降係數, 133, 157 Sedimentation equilibrium 平衡沈降, 134 Sedimentation velocity 速率沈降, 134 Selfish gene 自私的基因, 54 Separation gel, see Running gel Sephacel [商品名] 層析膠體, 8-9, 125, 202 Sephacryl [商品名] 層析膠體, 6-7, 119, 121-123, 201 Sephadex [商品名] 層析膠體, 119, 121-123, 125 Sepharose [商品名] 層析膠體, 119, 121-123, 125, 129, 138 Sequence analysis 序列分析, 159-160 Serine (Ser, S) 絲胺酸, 58-59, 82, 85, 88-89, 224-227 Serine proteases 絲胺酸蛋白脢, 82, 85-86, 146 SH3 domain, SH3 區塊, 224, 226 Signal peptide 信號胜汰, 65, 159, 224-227 Signal transduction 信息傳導, 89-90, 224-227 Silanization 矽化處理, 213-214 Silver staining 硝酸銀染色, 153-154, 212-214 Site-directed mutagenesis 人工定點突變, 96 Sodium azide (NaN3) 疊氮化鈉, 123, 144 Sodium dodecyl sulfate, see SDS Sodium thiosulfate 硫代硫酸鈉, 212, 215 Software, personal computer 個人電腦軟體, 30, 109 Solid matrix 固相擔體, 128 Solid phase 固定相, 95 Soluble starch 可溶性澱粉, 15, 143, 195, 215, 224-227 Spacer 間隔條, 204-205 Specific activity 比活性, 5, 15, 81, 138, Specificity 專一性, 68, 73, 86, 95, 128-130, 162 Spectrophotometer 分光光度計, 28, 109 Speed Vac [商品名] 離心真空濃縮, 136 Stability 酵素安定性, 145 Stacking gel 焦集膠體, 150, 153, 204, 206, 208 Staining methods 膠片染色方法, activity staining 活性染色, 19, 143-144, 215-216 Coomassie Brilliant Blue staining, CBR 染色, 153, 212, 221 PAS staining, PAS 醣染色, 153, 214-215 silver staining 硝酸銀染色, 153-154, 212-214 Standard proteins 標準蛋白質, isoelectric focusing 等電焦集法, 211 see Molecular weight standards Starch phosphorylase 澱粉磷解脢, activity assay 活性分析, 2-15, 19, 141-146, 195-196, 215-216 activity staining 活性染色, 4-13, 19, 143-144, 215-216 amino acid sequence 胺基酸序列, 226, 251 background information 背景資料, 224-228 dissertations 相關學位論文, 250 enzyme kinetics 酵素動力學, 14-15, 19, 196-198, 227 F50 片段, 224 L78 插入序列, 224-227 purification methods 純化方法, 199-204 Stationary phase 固定相, 118 Steady state theory 穩定狀態理論, 76-77 Steric specificity 立體專一性, 86 Stirred cell 攪拌加壓過濾濃縮槽, 135-136 Stock solution 貯藏溶液, 145 Stokes radius, Stokes 分子半徑, 119 Stopped-reaction 反應中止法, 141-142 Storage, reagent 試劑保存, 145 Streptavidin 鏈黴抗生物素蛋白, 218-220 Substrate 基質, 14-15, 76-96, 130, 141-146, 154, 195-198 Sulfa drug 磺胺藥, 82 Sulfosalicylic acid 磺基柳酸, 211 Sulfuric acid 硫酸, 195 Sumner, James B., 73 Svedberg unit 沉降係數 S, 133, 157 Sweet potato roots 甘藷塊根, 4, 5, 199, 225 Swiss-Prot [商品名] 瑞士蛋白質資料庫, 159 |
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T | |||
Tangential-flow 多層側流板濃縮, 135-136 TCA (trichloroacetic acid) 三氯醋酸, 142, 211 Teacher's guide 教師備忘錄, 239-244 Teaching assistant 助教, 237, 240, 245 TEMED (tetramethylethylenediamine) 四甲基乙二胺, 149, 152, 205 Tense form 緊縮型, 91 Tertiary structure 三級構造, 64, 66-67, 160, 225 TFA (trifluoroacetic acid) 三氟醋酸, 221 Thermacidophile 嗜酸熱菌, 52 Thin-layer electrophoresis (TLE) 薄層電泳, 148 Thiopropyl-Sepharose 6B 親和吸著劑, 129 Threonine (Thr, T) 穌胺酸, 58-59, 89 Thrombin 凝血脢, 87 Thyroglobulin 甲狀腺球蛋白, 156 TiterMax [商品名] 高效價佐劑, 163 TLC (thin layer chromatography) 薄層層析法, 118, 158 TLCK (tosyl-L-lysine chloromethyl ketone), trypsin 專一性抑制劑, 82, 145 Total volume (Vt) 總管柱體積, 120-121 TPCK (tosyl-L-phenylalanine chloromethyl ketone), chymotrypsin 專一性抑制劑, 82, 145 Transition state 過渡狀態, 76, 85, 96 Tris (tris(hydroxymethyl)aminomethane) 三羥甲基氫基甲烷, 25, 129, 139, 144-145, 158, 193, 199, 203, 205-206, 208, 217-221 Triton [商品名] 界面活性劑, 144 Trypsin 胰蛋白脢, 73, 82, 85, 88, 161 Trypsin inhibitor 胰蛋白脢抑制劑, 208, 211 Trypsinogen 胰蛋白脢原, 211 Tryptophan (Trp, W) 色胺酸, 58-59, 85, 158, 161 Turnover number, Kcat, 轉換數, 80 |
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U | |||
Ultracentrifugation 超高速離心法, 69, 94, 133-135, 157 Ultrafiltration 超微薄膜過濾法, 135-136, 138 Ultrasonication 超音波震盪, 113 Un-competitive inhibition 無競爭性抑制, 83 Uni-Uni reaction 單-單式雙基質反應, 81 Universal buffer 通用緩衝液, 144 Unsymmetrical carbon 不對稱碳, 57, 86 Urease 尿素脢, 73 |
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V | |||
Van der Waals bond 凡得瓦爾力, 55, 86 Velocity 反應速率, 77-80, 83, 141, 196-198 Vitamin B2 (riboflavin) 維生素 B2, 149 Vmax (maximum velocity) 最高催化速率, 15, 76-80, 83, 91, 141, 196-198, 227 |
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W | |||
Waring blender [商品名] 果汁機, 113, 199 Weekly schedule 每週時間表, 232 |
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XYZ | |||
X-ray crystallography, X 光結晶繞射分析, 69, 161, 225 Zero order reaction 零級反應, 79 Zone centrifugation 區帶離心, 134 Zone electrophoresis 帶狀電泳, 148 |
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建立日期:2001/4/20 更新日期:2003/05/23 © 版權所有