酵素化學實驗 Index 索 引 E-L | |||
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(3) 印刷本『酵素化學實驗』中並沒有收錄 核酸部份,因此也沒有編入本索引。 |
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E | |||
Eadie-Hofstee plot 動力學作圖法, 79 EDC (N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide HCl) 脫水劑, 129 Edman degradation, N-端胺基酸降解法, 70, 159, 167, 220-221 EDTA (ethylenediamine tetraacetic acid) 乙二胺四乙酸, 143, 144, 146, 206, 208, 218 Elastase 彈性蛋白脢, 85 Electroelution 電泳溶離, 10-11, 133, 204 Electronegativity 陰電性, 55 Electrophoresis 電泳, gel system 膠體系統, 150 principle 電泳原理, 147-153 see Disc-PAGE see Preparative electrophoresis see SDS-PAGE see Staining methods Electrostatic bond 離子鍵, 55 ELISA reader, ELISA 光度計, 28, 109, 193-198 ELISA, enzyme-linked immunosorbent assay 酵素免疫分析法, 95, 163-164 Elution speed 溶離速度, 123 Elution volume (Ve) 溶離體積, 120-121 Endorphin 腦啡, 88 Enzyme 酵素, 73-101 Enzyme complex 酵素複合體, 94 Enzyme electrode 酵素電極, 95, 142 Enzyme nomenclature 酵素命名, 73 Epoxy-activated Sepharose 6B 活化型親和吸著劑, 129 Epoxyl-Sepharose 6B 親和吸著劑, 129 ER (endoplasmic reticulum) 內質網, 52, 65 Ethylene glycol 乙二醇, 130 Ethylenediamine 乙二胺, 212 Exon 外顯子, 88 Extein 外顯蛋白, 88 |
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F | |||
Ferritin 鐵蛋白, 156 Ferro-sulfate molybdate 亞鐵硫酸鉬, 195 First order reaction 一級反應, 79 Flagella 鞭毛, 52 Flow charts 流程圖, enzyme kinetics 酵素動力學大綱, 77 enzyme purification stages 酵素純化階段與分析方法, 112 How to start? 如何開始? 238 immunization procedure 免疫及抗體純化流程, 163 protein isolation and microanalysis 蛋白質快速純化及微量分析, 166 protein technology 蛋白質科技, 165 X1-X6 各實驗單元流程圖, 2-14 Formate buffer 甲酸緩衝液, 144 FPLC (fast performance liquid chromatography) [商品名] 快速液相層析法, 131-132, 133, 156, 167 Fraction collector 分劃收集器, 121, 201 Fractogel TSK [商品名] 層析膠體, 132 Free radical 自由基, 149 Freezer 冷凍櫃, 109-110 Freezing 冷凍, 5, 109-110, 113, 145-146 French press, French 氏壓力研磨機, 113 Freund's adjuvant, Freund 氏佐劑, 163 Fusion protein 融合蛋白質, 95 |
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G | |||
Gamma turn, g 轉折, 66 Gas chromatography (GC) 氣相層析法, 118 GCG (Genetics Computer Group) [商品名] 序列分析軟體, 30, 159 Gel filtration 膠體過濾法, 6-7, 69, 118-124, 138, 156, 200-202, column packing 管柱裝填, 122-124, 156, 201-202 gel support 膠體介質, 119-120 molecular weight standard 分子量標準組, 156 sample application 樣本添加, 122 Gel regeneration 膠體再生, 123, 127 Gel support, gel matrix 膠體介質, 119-120 Gelatin 明膠, 218-220 Gene expression 基因表現, 93 Glass bead 玻璃珠, 113 Glc-1-P (glucose-1-phosphate) 葡萄糖一磷酸, 15, 143, 195-198, 215, 224-227 Glucagon 胰增糖素, 90 Glutamate dehydrogenase 麩胺酸去氫脢, 217 Glutamate transaminase 麩胺酸轉胺脢, 75 Glutamic acid (Glu, E) 麩胺酸, 58-59, 85, 158, 161 Glutamine (Gln, N) 麩醯胺酸, 58-59, 158 Glutaraldehyde 戊二醛, 212-213 Glyceraldehyde-3-P dehydrogenase 甘油醛三磷酸去氫脢, 75 Glycerol 甘油, 24, 145-146, 163, 206, 209-211 Glycine (Gly, G) 甘胺酸, 58-59, 66, 85, 150-151, 217 Glycogen phosphorylase 肝醣磷解脢, 74, 89, 224-228 Glycoprotein 糖蛋白, 67, 153, 214 Glyoxosome 乙醛酸循環體, 53 Golgi body 高爾基氏体, 52 Gradient gel electrophoresis 梯度電泳, 149, 156-157 Gradient formation 梯度製造, 8-9, 127, 133-134, 156-157, 202-204, 209-210 |
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H | |||
Halophile 嗜鹽菌, 52 Handerson-Hasselbalch equation 緩衝作用公式, 61 Hapten 半抗原, 162 Heavy metal 重金屬, 82 Hemocyanin 血藍蛋白, 162 Heparin 抗凝血素, 130 HEPES (N-2-hydroxyethylpiperazine-N'-2-ethane sulfonic acid), HEPES 緩衝液, 144 Heterotropic effect 異配體效應, 91 Hexokinase 六碳糖激脢, 67 HgCl2 (mercuric chloride) 氯化汞, 216 HIC (hydrophobic interaction chromatography) 疏水性層析法, 118, 130-131, 138 Histidine (His, H) 組胺酸, 58-59, 60, 74, 85-86, 89, 130 Histidine carboxylase 組胺酸去羧脢, 73 Holoenzyme 全脢, 74 Homogeneous enzyme 均質酵素, 94, 112, 166 Homogenizer 均質機, 113 Homotropic effect 同配體效應, 91 Hormone 荷爾蒙, 93 Horse radish peroxidase 山葵過氧化脢, 218, 220 HPLC (high performance liquid chromatography) 高效能液相層析法, 131-132, 133, 142, 156, 158, 161, 167 Human genome project 人類基因體計畫, 96, 167 Hydride, H- 離子, 75 Hydrogen bond 氫鍵, 55, 65-66, 68 Hydrogen peroxide (H2O2) 雙氧水, 53, 220 Hydrophobic bond (hydrophobic interaction) 疏水性引力, 55, 66, 68, 115-117, 130-131, 207, 212 |
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I | |||
Immobilized enzyme 固定化酵素, 95 Immunization 免疫流程, 163 Immunization procedure 免疫及抗體純化流程, 163 Immunoelectrophoresis 免疫電泳, 148 Immunoglobulin 免疫球蛋白, 139, 156, 163 Immunoprecipitation 免疫沉澱法, 164 Immunostaining 免疫染色, 12-13, 148, 155, 161, 164, 218-220 Inactivation 酵素失活, 146 Induced fit 誘生性契合, 86 Inhibitor 抑制劑, 82-83, 88, 138, 141, 143, 146 Intein 內隱蛋白, 88 Ion exchange 離子交換法, 2, 8-9, 69, 118, 124-127, 138, 142, 202-203, batch method 批次法, 127 buffer 緩衝液使用, 126 capacity 容量, 125 column operation 管柱操作, 125-127 counter ion 抗衡離子, 124 gradient elution 梯度溶離, 8-9, 127, 203 media 離子交換介質, 124-125 pecking order 離子取代順序, 124 regeneration 再生, 127 Ipomoea batatas [學名] 甘藷, 199 Irreversible inhibition 不可逆抑制, 82-83 Isoelectric focusing (IEF) 等電焦集法, 69, 138, 148, 155, 210-212 Isoelectric point, see pI Isoleucine (Ile, I) 異白胺酸, 58-59, 85 Isopycnic equilibration 等密度平衡, 134 |
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K | |||
Kav 管柱溶離常數, 120 Kcat, see Turnover number KDEL sequence, KDEL 內質網回收信號, 65, 159 Key word 關鍵字, 35 Kinetics 動力學, enzyme kinetics 酵素動力學, 76-80, 196 starch phosphorylase 澱粉磷解脢, 14-15, 19, 196-198, 227 Km (Michaelis-Menten constant) 動力學常數, 15, 76-80, 83, 141, 196-198, 227 |
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L | |||
Labeling 如何標示, 111 Laboratory layout 實驗室配置圖, 234-235 Laboratory, establishing 建立實驗室, 109-111 Lactate dehydrogenase 乳糖去氫脢, 156 Lactoglobulin 乳球蛋白, 211 Lentil lectin 扁豆凝集素, 211 Ligand 配體, 128 Like dissolves like 極性互溶律, 118 Lineweaver-Burk plot 雙倒數作圖法, 79, 83, 196-198 Liquid nitrogen 液態氮, 113 Little Blue Tank [商品名] 電泳溶離裝置, 203-204 Lock and key 鎖頭與鑰匙, 86 Lysine (Lys, K) 離胺酸, 58-59, 61, 85, 88, 140, 154, 161, 210, 212 Lysosome 溶脢体, 53 |
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建立日期:2001/4/20 更新日期:2003/05/23 © 版權所有